| 响应面法优化酿酒酵母谷胱甘肽的提取条件 |
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| 引用本文： | 郑丽雪,王立梅,梅艳珍,齐 斌. 响应面法优化酿酒酵母谷胱甘肽的提取条件[J]. 食品科学, 2010, 31(10): 60-63. DOI: 10.7506/spkx1002-6630-201010012 |
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| 作者姓名： | 郑丽雪 王立梅 梅艳珍 齐 斌 |
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| 作者单位： | 常熟理工学院生物与食品工程学院 |
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| 基金项目： | 苏州市科技发展计划应用基础研究项目(YJG0913)；江苏省高校“青蓝工程”中青年学术带头人项目 |
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| 摘 要： | 利用Box-Benhnken 中心组合设计和响应面法优化乙醇提取酿酒酵母胞内谷胱甘肽的条件。结果表明，谷胱甘肽最佳提取条件为乙醇体积分数43.7%、乙醇添加量7.03mL/0.1g 干酵母粉、抽提时间80min、谷胱甘肽提取率达最大为9.54mg/g。
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| 关 键 词： | 谷胱甘肽 乙醇 酿酒酵母 响应面分析 |
| 收稿时间： | 2009-08-03 |
Optimization of Glutathione Extraction from Saccharomyces cerevisiae Using Response Surface Methodology |
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| ZHENG Li-xue,WANG Li-mei,MEI Yan-zhen,QI Bin. Optimization of Glutathione Extraction from Saccharomyces cerevisiae Using Response Surface Methodology[J]. Food Science, 2010, 31(10): 60-63. DOI: 10.7506/spkx1002-6630-201010012 |
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| Authors: | ZHENG Li-xue WANG Li-mei MEI Yan-zhen QI Bin |
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| Affiliation: | College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China |
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| Abstract: | Box-Behnken design and response surface methodology were applied to maximize the glutathione yield from Saccharomyces cerevisiae extracted with alcohol. Results showed that the optimum conditions of glutathione extraction were as follows: ethanol concentration 43.7%, amount of added ethanol 7.03 mL/ 0.1 g yeast powder and extraction time 80 min. Under such optimal conditions, a maximal glutathione yield of 9.54 mg/g were obtained. |
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| Keywords: | glutathione ethanol Saccharomyces cerevisiae response surface analysis |
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