气相色谱-质谱法测定八角籽挥发油的化学组成

采用水蒸气蒸馏法提取八角籽挥发油，通过气相色谱- 质谱分析手段对广西三种八角籽挥发油的主要成分进行分析比较，用峰面积归一化法确定各组分的相对含量。通过比较研究表明：角花八角籽油的提取率最高，干枝的次之，大红的最低；在化学成分上，三种八角籽油的主要成分因品种的不同而有区别，从三种八角籽的挥发油中共分离出19 种化学成分，大红八角籽油的成分相对单一，主要成分为小茴香灵(49.77%)、α- 香柠檬烯(21.52%)、4- 甲氧基肉桂醛(14.80%)；角花八角籽油主要成分为小茴香灵(47.50%)、n- 棕榈酸(16.61%)、4- 甲氧基肉桂醛(4.96%)；而干枝八角籽油的成分较为复杂。

Chemical Component Analysis of Volatile Oil from Illicium verum Hook. f. Seed by Gas Chromatography-Mass Spectrometry

Volatile oil was extracted from Illicium verum Hook. f. seed by steam distillation and chemical components in volatile oil were analyzed using gas chromatography-mass spectrometry. The relative content of these components were calculated using peak area normalization method. The major chemical components in volatile oil were different in varieties. Results indicated that yield of volatile oil was the highest in Jiao-hua; and Gan-zhi took the second place and Da-hong exhibited the lowest yield. Totally 19 components were identified in star anise seed oils. Chemical components were determined to be feniculin (49.77%), α-bergamotene (21.52%) and 4-methoxycinnamaldehyde (14.80%) in Da-hong star anise seed oil; feniculin (47.50%), n-hexadecanoic acid (16.61%) and 4-methoxycinnamaldehyde (4.96%) in Jiao-hua star anise seed oil. The chemical components in volatile oil of Gan-zhi star anise seed were quite complex. These investigations provided experimental fundaments for exploitation of star anise seed.