| 高效毛细管电泳法在黄酮类化合物分析检测中的应用 |
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| 引用本文： | 周一鸣,周小理,崔琳琳. 高效毛细管电泳法在黄酮类化合物分析检测中的应用[J]. 食品科学, 2010, 31(20): 275-277. DOI: 10.7506/spkx1002-6630-201020055 |
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| 作者姓名： | 周一鸣 周小理 崔琳琳 |
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| 作者单位： | 1.上海应用技术学院香料香精技术与工程学院2.上海商学院食品工程系 |
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| 基金项目： | 国家自然科学基金项目(31071527)；上海市优秀青年基金项目(yyy09013)；上海市科委重点科技攻关项目(074905114)；“十一五”国家科技支撑计划项目子课题(2006BAD02B06/18) |
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| 摘 要： | 建立一种高效毛细管电泳(HPCE)法测定黄酮类化合物含量的方法。电解质溶液以20mmol/L 硼砂- 硼酸溶液(pH8.4)作为缓冲液，在25℃、20kV 压力条件下进行电泳，245nm 波长处检测，线性关系良好，在10min 内黄酮类化合物完全分离，符合定性研究和定量测定的要求。该方法具有较好的分离效果和良好的精密度，可应用于黄酮类化合物的测定。
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| 关 键 词： | 黄酮类化合物 高效毛细管电泳 |
| 收稿时间： | 2009-12-03 |
Application of High Performance Capillary Electrophoresis to the Determination of Flavonoid Compounds |
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| ZHOU Yi-ming,ZHOU Xiao-li,CUI Lin-lin. Application of High Performance Capillary Electrophoresis to the Determination of Flavonoid Compounds[J]. Food Science, 2010, 31(20): 275-277. DOI: 10.7506/spkx1002-6630-201020055 |
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| Authors: | ZHOU Yi-ming ZHOU Xiao-li CUI Lin-lin |
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| Affiliation: | 1. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 200235, China；2. College of Food Science, Shanghai Business School, Shanghai 200235, China |
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| Abstract: | A method was developed for the determination of flavonoid compounds using high performance capillary electrophoresis (HPCE). The electrolyte buffer used was 20 mmol/L borax/boracic acid solution (pH 8.4). Electrophoresis was performed at 25 ℃ and 20 kV voltage. The detection wavelength was set as 245 nm. The developed method displayed good linearity. A thorough chromatographic separation of flavonoid compounds was achieved within 10 min, which meets the requirements for qualification and quantification. This method giving good separation and having high precision is applicable for the determination of flavonoid compounds in practice. |
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| Keywords: | flavonoids high performance capillary electrophoresis (HPCE) |
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