野马追类黄酮的超声提取及其抗氧化活性研究

在单因素试验的基础上，采用Box-Behnken 试验设计优化超声提取野马追类黄酮的最佳工艺参数，并研究野马追类黄酮对DPPH 自由基的清除效果，最后与其他提取方法进行比较。结果表明：超声提取野马追类黄酮的最佳工艺参数为液料比11.3:1(mL/g)、超声温度58℃和超声时间29min，此时得率为1.142%；当野马追类黄酮质量浓度在6.556～32.78μg/mL 范围内，其对DPPH 自由基的清除率为46.90%～61.06%，且存在明显的量效关系。与其他提取方法比较，超声提取时间短、得率高。

Ultrasonic Extraction and Antioxidant Activity Evaluation of Flavonoids from the Whole Plant of Eupatorium lindleyanum DC

Ultrasonic extraction (UE) of flavonoids from the whole plant of Eupatorium lindleyanum DC was optimized using Box-Behnken design and response surface methodology on the basis of single factor experimental results. The scavenging effect of the extract against DPPH free radicals was also studied. Results indicated that the optimal extraction conditions of flavonoids from the whole plant of Eupatorium lindleyanum DC were liquid-to-ratio of 11.3:1, ultrasonic extraction temperature of 58 ℃ and ultrasonic treatment time of 29 min. The extraction rate of flavonoids under these optimal extraction conditions was 1.142%. It was calculated that the content of flavonoids in Eupatorium lindleyanum DC ranged from 6.556 to 32.78 μg/mL. The scavenging rate of flavonoids from Eupatorium lindleyanum DC against DPPH free radicals was between 46.90% and 61.06% in a dosedependent manner. Compared with other extraction techniques, UE exhibited higher yield and shorter extraction time.