黑葵花籽壳红色素的纯化及主要成分结构鉴定

选用8 种大孔树脂对黑色葵花籽壳红色素进行纯化，结果表明，AB-8 大孔吸附树脂最适合于色素的分离纯化，且通过静态吸附与解吸实验确定了最佳纯化的条件，纯化后色价提高5 倍，再经冷冻干燥得到了色素的精制品。定性分析及红外图谱、薄层色谱、高效液相色谱及质谱分析表明，葵花籽壳红色素为花色苷类色素，且主要成分为矢车菊-3- 葡萄糖苷。

Purification and Structural Identification of the Major Component of the Red Pigment from Black Sunflower Seed Shell

Eight kinds of macroporous adsorption resins were used for the static adsorption of the red pigment extract from black sunflower seed shell, and AB-8 type resin presented the highest adsorption ratio and was consequently selected for further investigations. The optimum conditions for the dynamic adsorption and desorption of the pigment extract were explored. After purification using AB-8 type resin under the optimized adsorption and desorption conditions, a 5-fold elevation in the color value of the pigment extract was obtained. Lyophilization gave a crystallized red pigment. The major components of the red pigment was identified to be ascyanidin-3-glucoside through qualitative tests and IR spectroscopic, thin layer chromatographic, HPLC and MS analyses.