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万寿菊花中反式叶黄素提取皂化工艺的优化
引用本文:王 闯,李大婧,宋江峰,刘春泉. 万寿菊花中反式叶黄素提取皂化工艺的优化[J]. 食品科学, 2010, 31(24): 95-101. DOI: 10.7506/spkx1002-6630-201024020
作者姓名:王 闯  李大婧  宋江峰  刘春泉
作者单位:1. 东北林业大学林学院2. 江苏省农业科学院农产品加工研究所3. 国家农业科技华东(江苏)创新中心农产品加工工程技术研究中心
基金项目:江苏省自然科学基金项目(BK2010467);中央高校基本科研业务费专项(DL10BA12)
摘    要:以万寿菊干花颗粒为原料,对万寿菊花中反式叶黄素进行同时提取皂化工艺研究。考察不同有机溶剂和用量、KOH- 乙醇溶液质量浓度和用量、提取皂化温度和时间等因素对反式叶黄素提取皂化效果的影响。在单因素试验基础上,采用响应曲面法对影响万寿菊花中反式叶黄素提取皂化效果的3 个主要因素即石油醚用量、KOH-乙醇溶液质量浓度和提取皂化温度进行优化,建立并分析各因素与反式叶黄素得率关系的数学模型,同时对反式叶黄素粗品进一步分离纯化。结果表明:采用有机溶剂法进行同时提取皂化的最佳工艺条件为石油醚用量42.6mL/g、温度58.8℃、KOH- 乙醇溶液质量浓度0.099g/mL,在此条件下反式叶黄素得率为1.499%。对反式叶黄素粗品进一步纯化,最终获得纯度达90.42% 的反式叶黄素晶体。

关 键 词:万寿菊  反式叶黄素  提取皂化  响应曲面法  纯化  
收稿时间:2010-03-02

Extraction and Saponification of Trans-lutein from Marigold Flowers
WANG Chuang,LI Da-jing,SONG Jiang-feng,LIU Chun-quan. Extraction and Saponification of Trans-lutein from Marigold Flowers[J]. Food Science, 2010, 31(24): 95-101. DOI: 10.7506/spkx1002-6630-201024020
Authors:WANG Chuang  LI Da-jing  SONG Jiang-feng  LIU Chun-quan
Affiliation:1. College of Forestry, Northeast Forestry University, Harbin 150040, China;2. Institute of Farm Products Processing, JiangsuAcademy of Agricultural Sciences, Nanjing 210014, China;3. Engineering Research Center for Agricultural Products Processing,National Agricultural Science and Technology Innovation Center in East China, Nanjing 210014, China
Abstract:Marigold (Tagetes erecta) flowers were used as raw materials to investigate the extraction and saponification of translutenin from marigold flowers. The effects of organic solvent amount, KOH-ethanol concentration and amount, extraction and saponification temperature and time on extraction and saponification rate of trans-lutenin from marigold flowers was explored. Based on the single factor experimental results, response surface experiments were used to optimize the extraction and saponification efficiency of trans-lutein and a mathematic model between the factors and extraction rate of trans-lutenin from marigold flowers was established. Meanwhile, crude trans-lutein was further purified. Results indicated that the optimal extraction parameters were raw material amount of 1.00 g, petroleum ether amount of 42.6 mL/g, KOH-ethanol concentration of 0.099 g/mL and extraction temperature of 58.8 ℃. Under the optimal extraction conditions, the extraction rate of trans-lutein was up to 1.499%. After the purification of trans-lutein, the content of trans-lutein crystal was up to 90.42%.
Keywords:Tagetes erecta  trans-lutein  extraction and saponification  response surface methodology  purification  
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