番茄及其制品中全反式番茄红素及bate-胡萝卜素含量的C30-HPLC内标法定量分析

目的：建立C30-HPLC 内标法对番茄及其制品中全反式番茄红素和β- 胡萝卜素进行同步定量检测方法。方法：色谱条件：固定相：YMC Carotenoid column C30 色谱柱 (4.6mm × 250mm，5μm)；流动相A：乙腈:甲醇(3:1，V/V)；流动相B：甲基叔丁基醚(MTBE)；流动相A 与B 中分别添加体积分数0.05% 的三乙胺；洗脱条件：B 在0～10min 内保持30%，在10～20min 内，B 由30% 线性增至90%，20～30min 内B 维持在90%；流速：1mL/min；色谱图检测波长470nm 和450nm；进样量：20μL；柱温：室温。内标物为全反式-8'- 阿朴-8'-β- 胡萝卜素醛。结果：此方法的最小定量限为0.01μg/mL。在0.3～0.6μg 范围内，内标物对全反式番茄红素和β-胡萝卜素的相对校正因子分别为1.36 和1.22，重复性RSD 分别为小于0.50%和0.70%，加样回收率均在99.00% 以上。结论：应用本方法可对番茄及其制品中的全反式番茄红素和β- 胡萝卜素进行定量分析。

Quantification of All-trans-lycopene and β-Carotene from Tomato and Its Products by Internal Standard Method on C30-HPLC

Objective: To establish a simultaneous determination method for all-trans-lycopene and β-carotene by internal standard on C30-HPLC. Methods: Stationary phase, YMCTM Carotenoid column C30 (4.6 mm×250 mm, SOD-5μm, Waters); mobile phase A, CH3CN-MeOH (3:1, V/V); mobile phase B, MTBE; both A and B with the addition of 0.05% triethylamine; elution, 30% B in 0－10 min, a linear increase of mobile phase B from 30% to 90% in 10－20 min and 90% mobile phase in 20－30 min; flow rate, 1 mL/min; PDA wavelength range, 300－600 nm; monitoring wavelength, 470 nm and 450 nm; injection volume, 20μL; column temperature, room temperature. Results: Minimum detection limit of this method was 0.01μg/mL. In the range of 0.3－0.6μg internal standard, relative correction factor of internal standard to all-trans-lycopene andβ-carotene were 1.36 and 1.22, respectively. The relative standard deviations of reproducibility experiments were 0.50% and 0.70%, respectively. The recovery rates of this method were higher than 99.00%. Conclusion: The established method is suitable for the determination for all-trans-lycopene and β-carotene in tomato and its products.