响应面法优化蓝莓叶黄酮的微波提取工艺

为确定蓝莓叶中黄酮提取的最佳工艺，以蓝莓叶为主要原料，在单因素试验基础上采用Box-Behnken 中心组合试验设计和响应面(RSM)分析法，建立微波提取黄酮的二次回归方程，并以黄酮提取率为响应值绘制响应面图和等高线图。考察乙醇体积分数、料液比、温度、微波功率及时间对黄酮提取率的影响。方差分析结果表明：乙醇体积分数、温度和微波功率对黄酮提取率影响显著；最佳工艺条件为提取温度72℃，乙醇体积分数64%，微波功率456W，在此工艺条件下黄酮提取率为4.232%。

Application of RSM to Microwave-assisted Extraction Optimization of Flavanoids from Blueberry Leaves

In order to obtain optimum conditions for extracting flavonoids from blueberry leaves under the assistance of ultrasonic, on the basis of single factor investigations, Box-Behnken experimental design was used to provide experimental data for establishing a regression model describing the extraction of flavonoids, and response surface and contour diagrams with the extraction yield of flavonoids as response were plotted for analyzing the pairwise interactive effects of ethanol concentration, material-to-liquid ratio, extraction temperature, microwave power and extraction duration on the response. The analysis of variances revealed that ethanol concentration, extraction temperature and microwave power had significant effect on the extraction yield of flavonoids. Extraction temperature of 72 ℃, ethanol concentration of 64% and microwave power of 456 W were found optimum. Under the optimized conditions, the maximum extraction yield of flavonoids was 4.232%.