芹菜素对3T3-L1前脂肪细胞增殖及分化作用研究

目的：研究芹菜素(apigenin，AP)对3T3-L1 前脂肪细胞增殖及分化的影响。方法：采用3T3-L1 前脂肪细胞，利用MTT 比色分析法检测不同质量浓度芹菜素对3T3-L1 前脂肪细胞增殖的影响；采用传统的鸡尾酒诱导剂诱导分化3T3-L1 前脂肪细胞，利用油红O 染色检测，通过比色分析法检测不同质量浓度芹菜素对3T3-L1 前脂肪细胞分化的影响。结果：30、50μg/mL 芹菜素相对于空白组和阳性对照组能够显著抑制3T3-L1 前脂肪细胞增殖和分化，提示其可能具有预防肥胖的作用。

Effect of Apigenin on the Proliferation and Differentiation of 3T3-L1 Preadipocytes

Objective: To investigate the effect of apigenin on proliferation and differentiation of 3T3-L1 preadipocytes. Methods: 3T3-L1 preadipocytes were used as target cells to explore the effect of apigenin on their proliferation measured by MTT assay. Besides, cell differentiation using differentiation cocktail, oil red O staining and spectrometric determination were carried out for investigating the effect of apigenin on the differentiation of 3T3-L1 preadipocytes. Results: Apigenin at 30μg/mL and 50μg/mL concentrations could inhibit the proliferation and differentiation of 3T3-L1 preadipocytes in comparison with the blank group and the positive control group (established using Dulbecco s modified Eagle s medium containing 0.5 mmol/L IBXM, 1μmol/L dexamethasone and 10μmol/L insulin). Conclusion: Apigenin appear to have potential for the prevention of obesity.