林蛙油对冷应激小鼠抗氧化能力的影响

研究林蛙油对冷应激小鼠抗氧化能力的影响，为林蛙油的合理开发和利用提供依据。本实验将40 只雄性昆明品系小鼠随机分为4 组：对照组、冷应激组、林蛙油5 倍剂量组和25 倍剂量组，每组10 只。对照组和冷应激组灌胃生理盐水，5 倍剂量组和25 倍剂量组灌不同剂量的林蛙油，每组连续灌胃28d。灌胃到第15 天，每天除对照组灌胃后置室温外，其他各组灌胃30min 后，置－ 10℃冰箱30min，连续14d，杀鼠，测血清和脑组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果表明，林蛙油可以增加小鼠血和脑组织中SOD 活性(p ＜ 0.01)，降低MDA 含量(p ＜ 0.01)，在低温条件下林蛙油能增强小鼠抗氧化能力。

Effects of Ovum Oil of Rana chensinensis on Antioxidant Capacity in Mice Exposed to Cold Stress

Objective: To study the effects of ovum oil of Rana chensinensis on antioxidant capacity in mice exposed to cold stress to lay a foundation for rational development and utilization of the oil. Methods: Forty male Kunming mice were randomly divided into four groups: control group, cold stress group, 5-time dose group of the oil and 25-time dose group of the oil, with 10 mice in each group. The two dose groups were subjected to a 28-day gastric perfusion, and the control group and the cold stress group were treated with the same amount of saline. A 30-min cold stress treatment to others except the control group mice began in －10 ℃ refrigerator 30 min after the gastric perfusion since the 15th day. The superoxide dismutase (SOD) activity and the MDA content in blood and brain tissues of newly sacrificed mice were measured. Results: The oil increased the activity of SOD (p < 0.01) in mice blood and brain tissues, and reduced the content of MDA (p < 0.01). Conclusion: The ovum oil of Rana chensinensis can enhance the antioxidant capacity in mice exposed to cold stress.