| 低聚糖阿魏酸酯含量的快速测定方法 |
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| 引用本文： | 赵阳阳,欧仕益,林奇龄,林海伟.低聚糖阿魏酸酯含量的快速测定方法[J].食品科学,2010,31(18):329-332. |
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| 作者姓名： | 赵阳阳 欧仕益 林奇龄 林海伟 |
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| 作者单位： | 1.暨南大学食品科学与工程系
2.华南理工大学轻工与食品学院 |
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| 基金项目： | 广东高校科技成果产业化重大项目(cgzhzd0709) |
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| 摘 要： | 分别以麦麸、玉米芯、玉米麸皮为原料，采用酶法制备低聚糖阿魏酸酯，探讨其快速检测方法，并通过高效液相色谱法验证该法的准确性。结果表明，采用Amberlite 树脂分离纯化可除去样品中的低聚糖(不含结合态的阿魏酸)、双波长分光光度法(345nm 和375nm)结合还原糖法可用于对低聚糖阿魏酸酯进行准确定量。
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| 关 键 词： | 低聚糖阿魏酸酯 阿魏酸 测定 双波长分光光度法 高效液相色谱法 |
| 收稿时间： | 2010-06-09 |
Rapid Determination of Feruloylated Oligosaccharides during Production |
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| ZHAO Yang-yang,OU Shi-yi,LIN Qi-ling,LIN Hai-wei.Rapid Determination of Feruloylated Oligosaccharides during Production[J].Food Science,2010,31(18):329-332. |
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| Authors: | ZHAO Yang-yang OU Shi-yi LIN Qi-ling LIN Hai-wei |
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| Affiliation: | 1. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China；
2. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China |
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| Abstract: | In this work, feruloylated oligosaccharides were prepared from each of three raw materials, namely, wheat bran, corn cob and corn bran, by enzymatic hydrolysis. A rapid method for determining feruloylated oligosaccharides was developed and verified by high performance liquid chromatography. Non-feruloylated oligosaccharides were removed by Amberlite resin chromatography, and the purified feruloylated oligosaccharide could be determined by using dual wavelength (DW) spectrophotometry (345 nm and 375 nm) coupled with the determination of reducing sugars after acidolysis. |
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| Keywords: | feruloylated oligosaccharides ferulic acid determination dual wavelength spectrophotometry highperformance liquid chromatography |
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