生物富硒对沼泽红假单胞菌G3菌株营养组分的影响

为了探讨生物富硒对沼泽红假单胞菌的营养品质的影响，对富硒沼泽红假单胞菌G3 菌株和普通沼泽红假单胞菌的一般营养成分、氨基酸、B 族维生素、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)进行测定。结果表明：富硒培养的沼泽红假单胞菌总硒含量为875.03μg/g，其中有机硒含量为847.42μg/g，有机硒转化率为96.85%。富硒沼泽红假单胞菌G3菌株的蛋白质、菌体可溶性糖和粗脂肪的含量分别比普通沼泽红单胞菌提高了9.10%、5.13%和42.86%，灰分含量则比普通菌降低了23.73%。富硒沼泽红假单胞菌菌体含有17 种氨基酸，普通菌体含有16 种氨基酸，富硒沼泽红假单胞菌菌体的多种氨基酸含量均高于普通菌，其中人体必需的蛋氨酸和赖氨酸分别比对照提高了100% 和9.2%。富硒沼泽红假单胞菌的VB2、VB6、烟酸和类胡萝卜素与对照组相比均明显提高，VB2 的含量提高了6.45%，VB6 的含量提高了5.77%，烟酸的含量提高了7.20%，类胡萝卜素含量提高了45.08%，VB1 与对照组相比下降了9.2%，富硒沼泽红假单胞菌菌体的SOD 酶和CAT 酶活性明显提高，分别比普通菌高158.43% 和54.51%。

Effect of Selenium Enrichment on Nutritional Composition of Rhodopseudomonas palustris G3

In order to explore the effect of selenium enrichment on nutritional compositions of Rhodopseudomonas palustris, general nutrients, amino acids, vitamin B family, nicotinic acid, β-caroteneare, superoxide dismutase (SOD) and catalase (CAT) in selenium-enriched Rhodopseudomonas palustris (sRP) and normal Rhodopseudomonas palustris (nRP) were compared. Results indicated that the contents of protein, soluble sugar and crude fat of sRP were higher than those of nRP, which exhibited an enhancement by 9.10%, 5.13% and 42.86%, respectively. Whereas, the ash content of sRP was lower than that of nRP with a decrease by 23.73%. In addition, totally 17 amino acids were detected in sRP, while only 16 amino acids was detected in nRP. Moreover, the contents of amino acids in sRP were much higher than those in nRP, especially for methionine and lysine with an increase by 100% and 9.2%, respectively. Vitamin B2, vitamin B6, nicotinic acid and carotenoid in sRP were 6.45%, 5.77%, 7.20% and 45.08%, which were also higher than those in nRP, respectively. However, the content of vitamin B1 in sRP was lower than nRP. The activities of SOD and CAT in sRP exhibited an enhancement of 158.43% and 54.51% when compared with those in nRP. Therefore, selenium-enrichment can improve nutritional quality of Rhodopseudomonas palustris.