| β-乳球蛋白IgE抗原决定簇的识别 |
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| 引用本文： | 丛艳君,任发政,云战友.β-乳球蛋白IgE抗原决定簇的识别[J].食品科学,2010,31(15):190-193. |
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| 作者姓名： | 丛艳君 任发政 云战友 |
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| 作者单位： | 1.北京工商大学化学与环境工程学院 2.中国农业大学食品科学与营养工程学院 3.内蒙古伊利实业集团股份有限公司 |
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| 基金项目： | “十一五”国家科技支撑计划项目(2006BAD04A06) |
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| 摘 要： | 以β- 乳球蛋白氨基酸序列为模板，错位合成β- 乳球蛋白多肽，以收集到的牛乳过敏患者血清为抗体，鉴定β- 乳球蛋白IgE 抗原决定簇，分析影响致敏性的关键氨基酸，探讨牛乳过敏机理。结果表明：β- 乳球蛋白IgE抗原决定簇有4 条，氨基酸序列分别为17～31、72～86、92～106、152～166，并且苏氨酸(AA20)、蛋氨酸(AA23)、天冬氨酸(AA27)是影响β- 乳球蛋白致敏性的关键氨基酸。说明特异性水解抗原决定簇或定点修饰氨基酸可以实现过敏原脱敏的目的。
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| 关 键 词： | 牛奶 β- 乳球蛋白 IgE 抗原决定簇 |
| 收稿时间： | 2009-11-24 |
Identification of IgE-binding Epitopes on β-lactoglobulin |
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| CONG Yan-jun,REN Fa-zheng,YUN Zhan-you.Identification of IgE-binding Epitopes on β-lactoglobulin[J].Food Science,2010,31(15):190-193. |
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| Authors: | CONG Yan-jun REN Fa-zheng YUN Zhan-you |
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| Affiliation: | 1. School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100048, China；
2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China；
3. Inner Mongolia Yili Industrial Group Co. Ltd., Hohhot 010080, China |
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| Abstract: | A series of overlapping decapeptides (offset by five amino acids) were synthesized on the basis of the amino acid sequence of β-lactoglobulin. IgE-binding epitopes were identified using individual sera from cow milk allergic patients. In addition, we also identified critical amino acids (AAs) for IgE binding. The results showed that four IgE-binding epitopes were identified, which located in the sequences of AA 17 － 31, AA 72 － 86, AA 92 － 106 and AA 152 － 166, respectively. Threonine, methionine and aspartic acid were the critical amino acids for allergenicity of β-lactoglobulin. |
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| Keywords: | cow milk β-lactoglobulin IgE epitope |
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