| 利用荧光假单胞菌固定化细胞生产2- 酮基-D- 葡萄糖酸 |
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| 引用本文： | 陈 萍,苗晓燕,张筱梅,周 强,孙文敬.利用荧光假单胞菌固定化细胞生产2- 酮基-D- 葡萄糖酸[J].食品科学,2010,31(21):258-261. |
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| 作者姓名： | 陈 萍 苗晓燕 张筱梅 周 强 孙文敬 |
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| 作者单位： | 1.保定学院生化系
2.百勤异VC 钠有限公司 3.江苏大学食品与生物工程学院 |
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| 基金项目： | 江苏大学科研启动基金项目(08JDG029)；江西省主要学科学术和技术带头人培养计划项目(2008DD00600)；
江西省科技支撑计划项目(赣财教[2008]147号) |
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| 摘 要： | 以海藻酸钠为载体固定荧光假单胞菌(Pseudomonas fluorescens)AR4，考察固定化细胞发酵生产2- 酮基-D-葡萄糖酸的条件。结果表明：利用5.0% 海藻酸钠制备的固定化细胞颗粒稳定性好，可重复利用7 次；发酵培养基中玉米浆的最适质量浓度为0.75g/100mL，固定化细胞的适宜发酵温度为30～36℃；在适宜的培养条件下，固定化细胞与游离细胞的2- 酮基-D- 葡萄糖酸产量及糖酸转化率基本相同，分别可达13.5g/100mL 和90.0% 左右。
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| 关 键 词： | 固定化细胞 荧光假单胞菌 2- 酮基-D- 葡萄糖酸 海藻酸钠 发酵 |
| 收稿时间： | 2010-08-30 |
Production of 2-Keto-D-Gluconic Acid Using Immobilized Pseudomonas fluorescens AR4 |
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| CHEN Ping,MIAO Xiao-yan,ZHANG Xiao-mei,ZHOU Qiang,SUN Wen-jing.Production of 2-Keto-D-Gluconic Acid Using Immobilized Pseudomonas fluorescens AR4[J].Food Science,2010,31(21):258-261. |
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| Authors: | CHEN Ping MIAO Xiao-yan ZHANG Xiao-mei ZHOU Qiang SUN Wen-jing |
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| Affiliation: | 1. Department of Biochemistry, Baoding University, Baoding 071000, China；2. Parchn Sodium Isovitamin C Co. Ltd., Dexing
334221, China；3. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China |
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| Abstract: | The cells of Pseudomonas fluorescens AR4 were immobilized by sodium alginate, and the optimization of fermentation conditions for producing 2-keto-D-gluconic acid by immobilized cells were investigated. The results showed that 5.0% of sodium alginate was the optimal carrier for immobilizing Pseudomonas fluorescens AR4 to give a high yield, and the immobilized cells could be reused for 7 times. The optimal concentration of corn steep liquor in fermentation medium was 0.75g/100 mL, and fermentation temperature was between 30 ℃ and 36 ℃. Under the optimal conditions, immobilized cells and free cells could give similar yield and conversion rate of 13.5 g/100 mL and 90.0%, respectively. |
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| Keywords: | immobilized cell Pseudomonas fluorescens 2-keto-D-gluconic acid sodium alginate fermentation |
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