氯霉素分子印迹聚合物微球的制备及色谱性能评价

以氯霉素(CAP)为模板分子、甲基丙烯酸N,N- 二乙基氨基乙酯(DEAEM)为功能单体、二甲基丙烯酸乙二醇酯(EGDMA)为交联剂、聚苯乙烯微球作为种球，采用两步溶胀聚合的方法制备得到了粒径均一的分子印迹聚合物微球(MIPMs)。结果表明：扫描电镜观察显示其表面为多孔结构；通过孔容和孔径研究表明，与非印迹聚合物微球(NIPMs)相比，MIPMs 具有更大的孔径和孔体积；进一步通过液相色谱对其选择性能进行评价，可知氯霉素、甲砜霉素、氟甲砜霉素在MeCN/Water/H3PO4(30:70:0.1，V/V)流动相条件下，3 种物质达到了较好的分离效果，获得的MIPMs 对3 种物质具有较好的选择性能。

Preparation and Chromatographic Evaluation of Molecularly Imprinted Polymer Microspheres for Chloramphenicol

A uniformly sized molecularly imprinted polymer microspheres (MIPMs) for chloramphenicol (CAP) has been prepared by a two-step swelling and polymerization method using 2-(Diethylamino) ethyl methacrylate (DEAEM), ethylene glycol dimethacrylate (EGDMA) and polystyrene microspheres as the functional monomer, cross-linker and seed particles, respectively. The porous surface of MIPMs could be clearly observed under scanning electron microscope (SEM). Furthermore, the MIPMs had a larger pore volume and pore diameter measured using BELSORP-miniII than the non-imprinted polymer microspheres (NIPMs) did. The selectivity of MIPMs was assessed by chromatographic method, and CAP, thiamphenicol (TAP) and florfenicol (FF) could reach a stronger separation on the MIPMs with a MeCN/water/H3PO4 mixture (30:70:0.1,V/V) as the mobile phase.