高效液相色谱法测定罗汉果中VC的含量

目的：建立HPLC 色谱法测定罗汉果中VC 含量的方法。采用5g/L 草酶对罗汉果浸提15min，对提取液中VC 进行检测，具体方法：采用SinoChrom ODS-BP(250mm × 4.6mm，5μm)分离；流动相为体积分数0.05% 磷酸溶液- 甲醇(98:2，V/V)；流速1.0mL/min；采用二极管阵列多波长检测器确定VC 的最大吸收波长为242nm；测定温度25℃；进样量10μL。结果表明：VC 溶液0.08～0.50mg/mL 范围线性关系良好，相关系数r=1；回收率为99.3%，RSD 为1.93%。结论：该方法操作简便快速，精密度和稳定性好，适用于罗汉果VC 含量的测定。

Determination of the Content of Vitamin C in Siraitia grosvenorii Fruits by HPLC

Objective: To set up a HPLC method for the determination of the content of vitamin C in the fruits of Siraitia grosvenorii. Methods: The chromatographic separation was achieved on SinoChrom ODS-BP (250 mm×4.6 mm, 5μm) held at 25 ℃ using a mixture composed of 0.05% H3PO4 and CH3OH (98:2, V/V) as the mobile phase at a flow rate of 1.0 mL/min. The diode array detector wavelength was set at 242 nm. The injection volume used was 10μL. Results: The developed calibration curve of vitamin C displayed good linearity over the concentration range from 0.08 to 5.0 mg/mL, with a correlation coefficient equaling 1. The average recovery of five replicates for vitamin C was 99.3% with 1.93% relative standard deviation (RSD). Conclusion: This method is simple, quick, precise and stable, and can used for determining the content of vitamin C in the fruits of Siraitia grosvenorii.