黄粉虫抗氧化活性肽的分离纯化研究

为制备具有抗氧化活性的黄粉虫生物活性肽，采用双酶水解黄粉虫蛋白粉，对酶解产物进行葡聚糖SephadexG-25 凝胶柱层析和阳阴离子交换柱层析分离纯化，并对其各组分分子量分布及其抗氧化性进行研究，对抗氧化活性最佳的肽段进行氨基酸组成分析。结果显示，纯化得到的抗氧化多肽对羟自由基和超氧阴离子自由基具有很强的清除作用，清除率可分别达到74.20% 和87.32%。

Separation and Purification of Antioxidant Peptides from Yellow Mealworm

In order to prepare antioxidant peptides from yellow mealworm, yellow mealworm protein powder was hydrolyzed through dual enzyme system and hydrolysates were purified using Sephadex G-25 and ion-exchange column chromatography. The distribution of molecular weight and antioxidant capability of each component were determined. Meanwhile, amino acid composition of the optimal antioxidant peptide was also analyzed. Results indicated that antioxidant peptides from yellow mealworm exhibited strong scavenging capability to hydroxyl free radicals and superoxide free radicals with the scavenging rates reaching 74.20 % and 87.32 %, respectively.