响应面法优化离子交换法固定化β-D-呋喃果糖苷酶

以大孔阴离子树脂D311 为载体，对日本曲霉来源的β-D- 呋喃果糖苷酶进行离子交换法固定化。研究温度、pH 值、时间、游离酶液酶活力对固定化效果的影响，并在此基础上运用响应面法对固定化条件进行优化。结果表明，最佳固定化条件为：室温、pH6.6、固定化时间4h、游离酶液酶活力为900U/mL，在此条件下，固定化β-D- 呋喃果糖苷酶生产的低聚果糖产量可达58.16%。

Optimization of Ion Exchange Immobilization of β -Fructofuranosidase Using Response Surface Methodology

D311 type macroporous anion exchange resin was used as a carrier for the immobilization of β-fructofuranosidase derived from Aspergillus japonicus. The effects of immobilization time and temperature, pH and enzyme activity of free β-fructofuranosidase solution on the catalytic ability of immobilized β-fructofuranosidase were investigated by single factor method and based on this, except immobilization temperature, three other conditions were optimized using response surface methodology. The optimum immobilization conditions were determined as follows: enzyme activity of free β-fructofuranosidase solution 900 U/mL and pH 6.6 for 4 h immobilization at room temperature, and the yield of fructooligosaccharides using immobilized β-fructofuranosidase under these conditions was 58.16%.