山楂多酚含量的反相高效液相色谱法测定

建立同时测定山楂中绿原酸、表儿茶素和芦丁3 种酚类物质的反相高效液相色谱方法，并研究这三种酚类物质在山楂干果果肉、干果果核和鲜果果肉中的含量。经测定，表儿茶素在山楂鲜果果肉中含量最高，达到(1.661 ± 0.024) mg/g；绿原酸和芦丁在山楂干果果肉中含量最高，分别达到(0.550 ± 0.002) mg/g 和(0.498 ± 0.002) mg/g。本方法样品处理简单，具有良好的重现性和线性，相关性系数均达到0.999，绿原酸、表儿茶素和芦丁的回收率分别为91.8%～110.9%、94.6%～107.9% 和94.2%～108.8%；检出限分别为0.14、0.37 和0.68μg/ml (S/N ＝ 3)。

RP-HPLC Determination of Total Phenolic Compounds in Hawthorn

A simple, rapid and sensitive reversed-phase high-performance liquid chromatography method (RP-HPLC) was developed for the simultaneous determination of chlorogenic acid, epicatechin and rutin in hawthorn. The contents of these phenolic compounds in pulp and stone of dry fruit and pulp of fresh fruit were determined. The pulp of fresh hawthorn fruit had a highest epicatechin content of (1.661 ± 0.024) mg/g and the contents of chlorogenic acid and rutin in pulp of dry hawthorn fruit both were the highest, reaching (0.550 ± 0.002) mg/g and (0.498 ± 0.002) mg/g, respectively. The regression equations for the three analytes presented good linearity and all the correlation coefficients were over 0.999. This method had good precision and repeatability. The spike recoveries for chlorogenic acid, epicatechin and rutin ranged from 91.8% to 110.9%, 94.6% to 107.9% and 94.2% to 108.8%, respectively, and the detection limits were 0.14, 0.37 and 0.68 μg/ml, separately.