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真姬菇液体菌种培养基筛选和摇瓶发酵条件的研究
引用本文:王丽娟,刘林德,卜庆梅,王爱云,王淑芳. 真姬菇液体菌种培养基筛选和摇瓶发酵条件的研究[J]. 食品科学, 2010, 31(17): 323-326. DOI: 10.7506/spkx1002-6630-201017072
作者姓名:王丽娟  刘林德  卜庆梅  王爱云  王淑芳
作者单位:鲁东大学生命科学学院
摘    要:采用摇瓶培养法对真姬菇液体菌种培养基筛选和发酵条件进行研究。研究表明,真姬菇液体菌种最佳组合培养基为:葡萄糖3g/100mL、玉米淀粉1g/100mL、黄豆粉1.5g/100mL、(NH4)2SO4 0.3g/100mL、KH2PO4 0.2g/100mL、MgSO4·7H2O 0.1g/100mL 和VB1 10mg/L。真姬菇摇瓶发酵最佳条件为:起始pH 值为6.0,500mL 锥形瓶装液量140~150mL,摇床转速180r/min,温度26℃,二级摇瓶发酵时间72h,接种量10%~12%。

关 键 词:真姬菇  培养基筛选  发酵条件  
收稿时间:2010-06-27

Screening of Optimal Liquid Medium for Hypsizigus marmoreus and Optimization of Its Shake Flask Incubation Conditions
WANG Li-juan,LIU Lin-de,BU Qing-mei,WANG Ai-yun,WANG Shu-fang. Screening of Optimal Liquid Medium for Hypsizigus marmoreus and Optimization of Its Shake Flask Incubation Conditions[J]. Food Science, 2010, 31(17): 323-326. DOI: 10.7506/spkx1002-6630-201017072
Authors:WANG Li-juan  LIU Lin-de  BU Qing-mei  WANG Ai-yun  WANG Shu-fang
Affiliation:School of Life Sciences, Ludong University, Yantai 264025, China
Abstract:In this study, medium composition and process conditions for the liquid-state incubation of Hypsizigus marmoreus in a shake flask were explored. The optimal medium composition was found to consist of 3 g/100 mL glucose, 1 g/100 mL corn starch, 1.5 g/100 mL soybean cake, 0.3 g/100 mL (NH4)2SO4, 0.2 g/100 mL KH2PO4, 0.1 g/100 mL MgSO4·7H2O and 10 mg/L Vitamin B1. Initial pH of 6.0, medium volume contained in a 500 mL flask of 140-150 mL, shaking speed of 180 r/min, incubation temperature of 26 ℃, incubation time of 72 h and inoculum size of 10%-12% were found optimal.
Keywords:Hypsizigus marmoreus  medium selection  incubation conditions  
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