响应面法优化闪式提取牛蒡苷工艺优化

采用响应面分析法优化牛蒡苷闪式提取的工艺条件。以牛蒡苷得率为指标，在单因素试验基础上，应用Box-Benhnken 中心组合设计试验，对提取工艺影响较大的乙醇体积分数、提取时间和液料比3 个工艺参数进行优化。最佳工艺条件为：乙醇体积分数83%、提取时间93s、液料比17:1(mL:g)、牛蒡子粒径≤ 1.0mm，在此工艺条件下，牛蒡苷得率为31.62mg/g，与预测值31.72mg/g 基本相符。闪式提取法是一种快速有效的提取牛蒡苷的方法。

Optimization of Homogenate Extraction for Arctiin from Arctium lappa L. by Response Surface Methodology

Response surface analysis (RSA) methodology was used for optimizing homogenate extraction processing of arctiin from Arctium lappa L. Based on single-factor experiments, the effects of ethanol concentration, extraction time and liquid-material ratio on extraction rate of arctiin were explored by Box-Benhnken central composite design method. The optimal extraction conditions were ethanol concentration of 83%, extraction time of 93 s, liquid-material ratio of 17:1 (mL/g) and particle size of less than 1.0 mm. Under these optimal extraction conditions, the actual yield of total arctiin was 31.62 mg/g, which was consistent with the predicted yield of 31.72 mg/g. Results indicated that homogenate extraction was an effective method to extract arctiin from Arctium lappa L.