血红素铁脂质体的制备

目的：制备血红素铁脂质体。方法：采用乙醇注入法及旋转薄膜- 超声法制备，再经过不同孔径的滤膜得到不同粒径的血红素铁脂质体，在原有技术的基础上优化血红素铁脂质体各组分的制备工艺，应用紫外分光光度计测定血红素铁的含量，计算出血红素铁脂质体的包封率，用差示扫描量热法检测血红素铁脂质体各组成物质的相变过程。结果：两种方法都能够得到较为理想的脂质体，血红素铁:胆固醇:卵磷脂质量比范围为(0.1～200):(1～10):(10～100)。结论：所得血红素铁脂质体呈均一大单室型，通过0.8μm 的滤膜后有效粒径为0.804μm，最大包封率36%。

Preparation of Heme Iron Liposomes

Ethanol injection and rotary film-ultrasonic methods were applied to prepare liposomes with heme iron in this study. Heme iron content and entrapment efficiency in liposomes obtained were measured by spectrophotometry, liposome morphology was observed under optical microscope and transmission electron microscope, and phase changes in cholesterol, lecithin and heme iron were determined by differential scanning calorimetry (DSC). Results showed that the heme iron liposomes prepared by the two methods were basically identical in most indicators. The mass ratio of hemin to cholesterol to phospholipids was in range of (0.1-200): (1-10): (10-100). The liposomes obtained are uniform and big single-chamber particles with an effective diameter of 0.804μm and the maximal entrapment efficiency is 36%.