爬山虎籽中原花青素提取纯化工艺及含量测定

设计正交试验探讨从爬山虎籽中提取纯化原花青素的工艺。结果表明：乙醇浸提的最佳浸提工艺为乙醇体积分数60%、提取温度70℃、提取时间90min、料液比1:10(g/mL)，在最佳浸提工艺条件下，从爬山虎籽中提取原花青素得率1.407%；大孔吸附树脂分离纯化原花青素的结果表明，AB-8 树脂适合精制爬山虎籽中原花青素，柱分离条件为上样液质量浓度为4mg/mL、洗脱液为体积分数30% 的乙醇溶液、洗脱流速2.0BV/h。精制后的原花青素经HPLC 检测其纯度达88.0%。

Extraction and Purification of Procyanidins from the Seeds of Parthenocissus tricuspidata

In this study, we focused on the orthogonal array optimization of ethanol extraction of Parthenocissus tricuspidata seed procyanidins, followed by the investigations on macroporous resin purification of the crude procyanidin extract. The optimum conditions for ethanol extraction of Parthenocissus tricuspidata seed procyanidins were determined to be: ethanol concentration 60% (V/V), extraction temperature 70 ℃, extraction time 90 min and solid-to-liquid ratio 1:10 (g/mL); the procyanidin yield was 1.407% under such conditions. It was showed that AB-8 resin was more suitable for procyanidin purification and that the optimum purification conditions were as follows: sample concentration, 4 mg/mL; desorption solvent, 30% ethanol; and desorption flow rate, 2.0 BV/h. The purity of the final product was determined by HPLC to be 88.0%.