酶法合成没食子酸叶黄素酯的研究

以叶黄素和没食子酸为原料，在脂肪酶Novozym435 的催化下，合成没食子酸叶黄素酯，并用IR 对其结构进行表征。研究脂肪酶催化叶黄素与没食子酸合成没食子酸叶黄素酯的影响因素，考察酶量、溶剂、体系水分、温度、时间等因素对酯化反应的影响。结果表明，适宜的工艺条件为酶用量10mg/mL、溶剂为氯仿、初始加水量60mg/g、温度40℃、反应时间30h，在上述条件下没食子酸转化率可达81.8%。

Lipase-catalyzed Synthesis of Gallic Acid Lutein Ester

Lutein and gallic acid were used as the raw materials to synthesize gallic acid lutein ester through the catalysis of Lipozyme Novo 435. The structure of the synthesized product was determined by IR. The effects of enzyme load, solvent, and water content, reaction temperature and reaction time on the esterification were investigated. The optimal reaction conditions were determined as follows: enzyme load, 10 mg/mL; water content, 60 mg/g; reaction medium, chloroform; reaction temperature, 40 ℃; and reaction time, 30 h. The conversion rate of gallic acid was up to 81.8% under these conditions.