| UPLC/Q-TOFMSE法快速鉴定奋乃静在人胆汁中的代谢物 |
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| 引用本文： | 谢岑,陈笑艳,邓泮,尤天庚,钟大放.UPLC/Q-TOFMSE法快速鉴定奋乃静在人胆汁中的代谢物[J].质谱学报,2009,30(Z1):91-94. |
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| 作者姓名： | 谢岑 陈笑艳 邓泮 尤天庚 钟大放 |
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| 作者单位： | 1. 中国科学院上海药物研究所，上海 201203；2. 上海市东方医院，上海 200120 |
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| 摘 要： |
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Rapid Identification of Perphenazine Metabolites in Human Bile by UPLC/Q-TOF MSE |
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| XIECen,CHENXiao-yan,DENGPan,YOUTian-geng,ZHONGDa-fang.Rapid Identification of Perphenazine Metabolites in Human Bile by UPLC/Q-TOF MSE[J].Journal of Chinese Mass Spectrometry Society,2009,30(Z1):91-94. |
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| Authors: | XIECen CHENXiao-yan DENGPan YOUTian-geng ZHONGDa-fang |
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| Affiliation: | 1. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
2. Shanghai East Hospital, Shanghai 200120, China |
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| Abstract: | The metabolites of perphenazine in human bile were rapid investigated employing UPLC/Q-TOF MSE (where E represents energy) technique. The bile samples were collected after dosing to human and purified through acetonitrile protein precipitation. Data collection was done by UPLC/Q-TOF MSE. According to the molecule formular educed from the measured accurate mass, mass defect filter(MDF) technique and generic dealkylation tool were used to screen the metabolites. Their structures were elicited by comparing the fragmentation patterns between parent drug and metabolites. All the metabolites has chlorine atom isotope cluster. After oral administration of perphenazine to the patient, 29 metabolites including 16 phase I and 13 phase II metabolites are detected in human bile. The major metabolic pathways of perphenazine in human are hydroxylation, N-dealkylation, desaturation, sulfation and glucuronate conjugation. |
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| Keywords: | perphenazine ultraperformanceliquidchromatography/quadrupoletime-of-flightmassspectrometer(UPLC/Q-TOFMS) metabolite |
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