香菇中40种农药残留量气相色谱法测定

目的：建立香菇中24 种有机氯和拟除虫菊酯、16 种有机磷农药残留量的气相色谱测定方法。方法：称取样品于聚四氟乙烯离心管中，乙腈高速匀质提取，上清液浓缩至近干，过Carbon/NH2 SPE 净化柱，正己烷和丙酮定容，用GC-ECD 和GC-FPD 外标法定量。结果：研究香菇中40 种农药的检测方法，方法的线性范围为0.016～0.80μg/mL，相关系数均大于0.999，回收率为73%～113%，重复性RSD 为3.2%～5.9%，检出限为0.0005～0.05mg/kg。结论：此气相色谱法可以应用于香菇等食用菌的多农药残留量检测。

GC Method for the Determination of Forty Pesticide Residues in Shiitake Mushroom

Objective: To develop a capillary GC method for the determination of the residues of 24 organochlorines or synthetic pyrethroid and 16 organophosphorus pesticides in shiitake mushroom. Methods: Samples were weighed into Teflon centrifuge tubes and high-speed homogenized with acetonitrile, and the supernatant was then condensed until almost dry and applied to a carbon/NH2 SPE column for cleanup. After recondensation until almost dry, the eluate was rediluted with n-hexane before being injected into the analytical instrument. Electron capture detector was used to detect organochlorines and pyrethroids and flame photometric detector to detect organophosphorus. Using the external standard method, quantitation was carried out. Results: The linear range of the proposed method was between 0.016μg/mL and 0.80 μg/mL, with a correlation coefficient of larger than 0.999, and its recovery, repeatability RSD and detection limit varied from 73.0% to 113%, from 3.2% to 5.9% and from 0.0005 to 0.05 mg/kg. Conclusion: A simple, sensitive and reproducible method applicable to the determination of pesticide multiresidues in shiitake mushroom and other species of mushroom has been developed.