n-3多不饱和脂肪酸饮食对小鼠肥胖及相关细胞因子的影响

目的：研究n-3 多不饱和脂肪酸(polyunsaturated fat acids，PUFAs)饮食对小鼠肥胖程度及相关细胞因子血清水平的影响，探讨其作用机理。 方法：将健康小鼠分为高饱和脂肪酸饮食组、n-3 多不饱和脂肪酸饮食组和对照组，记录每周小鼠进食量和体质量，实验结束时用ELISA 法检测小鼠血清增食因子A(orexin-A)、抵抗素(resistin)、脂联素(adiponectin)水平。 结果：n-3 多不饱和脂肪酸饮食组小鼠与高饱和脂肪酸饮食组小鼠相比，肥胖程度明显降低 (P ＜ 0.05)，血清的增食因子A 水平明显降低(P ＜ 0.05)，血清的抵抗素水平明显升高(P ＜ 0.05)，血清的脂联素水平升高但无统计学差异；n-3 多不饱和脂肪酸饮食组小鼠的各项指标和对照组相比均无统计学差异。结论：n-3 多不饱和脂肪酸饮食与饱和脂肪酸饮食相比，可抑制肥胖的发生和发展。

Effect of n-3 PUFA-containing Diet on Obesity and Obesity-related Cell Factors in Mice

Objective: To determine the effect of n-3 PUFA diet on obesity and obesity-related cell factors in mice. Methods: A total of 36 healthy female mice were randomly divided into three groups: high fat diet group, n-3 PUFA-containing diet group and control group. Body weight and food intake were recorded once a weed during the trail period and the levels of serum adiponectin, orexin-A and resistin were determined by ELISA method at the end of the trial. Results: The adiposity and serum orexin-A level in the n-3 PUFA-containing diet group were significantly lower than that in the high fat diet model group (P ＜0.05), and the level of serum resistin in the n-3 PUFA-containing diet model group was significantly higher than that in the high fat diet model group (P ＜ 0.05). Conclusion: An obvious reduction on the development of adiposity was observed in mice fed n-3 PUFA-containing diet.