| 响应面法优化碱法提取林蛙卵团中粗多糖的工艺研究 |
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| 引用本文： | 刘晓丹,郑明珠,刘景圣.响应面法优化碱法提取林蛙卵团中粗多糖的工艺研究[J].食品科学,2010,31(22):96-100. |
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| 作者姓名： | 刘晓丹 郑明珠 刘景圣 |
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| 作者单位： | 吉林农业大学食品科学与工程学院 |
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| 基金项目： | 国家自然科学基金项目(30671536) |
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| 摘 要： | 以林蛙卵团胶状物为原料，采用碱法对粗多糖进行提取，在单因素试验基础上，采用响应面法对NaOH溶液浓度、料液比、提取温度及提取时间进行优化。结果表明，碱法提取林蛙卵团胶状物粗多糖的最佳工艺条件为NaOH 溶液浓度1.2mol/L、料液比1:8(g/mL)、浸提温度56℃、浸提时间73min。在此条件下林蛙卵团胶状物粗多糖含量可达24.01mg/g。
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| 关 键 词： | 林蛙 卵团胶状物 多糖 响应面 |
| 收稿时间： | 2010-05-26 |
Optimization of Alkaline Extraction Conditions for Crude Polysaccharides from Rana Egg Jelly by Response Surface Methodology |
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| LIU Xiao-dan,ZHENG Ming-zhu,LIU Jing-sheng.Optimization of Alkaline Extraction Conditions for Crude Polysaccharides from Rana Egg Jelly by Response Surface Methodology[J].Food Science,2010,31(22):96-100. |
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| Authors: | LIU Xiao-dan ZHENG Ming-zhu LIU Jing-sheng |
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| Affiliation: | College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | Rana egg jelly was used as the raw material to extract polysaccharides by an alkaline extraction method. Based on single factor experiments, NaOH concentration, material-liquid ratio, extraction temperature and extraction time during extraction process were optimized by response surface methodology. Results exhibited the best extraction conditions were NaOH concentration of 1.2 mol/L, material-liquid ratio of 1: 8, extraction temperature of 56 ℃ and extraction time of 73 min. The extraction rate of crude polysaccharides can reach up to 24.01 mg/g. |
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| Keywords: | Rana egg jelly polysaccharides response surface methodology |
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