| Abstract: | Schizochytrium sp. is considered as a promising alternative commercial source of docosahexaenoic acid (DHA), but the production is hindered by the high feedstock cost. In this study, inulin is used as a cheap and readily available feedstock for Schizochytrium sp. ATCC 20888 to produce DHA. The strain could not utilize inulin directly and therefore inulin first needed to be hydrolyzed. Compared with the acidic hydrolyzate by HCl and hydrolyzate by endo‐inulinase, the hydrolyzate by exo‐inulinase serves as the most effective carbon source for microalgal growth. Hydrolysis of inulin by exo‐inulinase is further optimized, and up to 97.8% of inulin conversion is obtained under the optimal conditions of 40 °C, pH 7.0, substrate concentration of 80 g L?1 and exo‐inulinase loading of 2 g kg?1 substrate for 12 h. The resulting hydrolyzate containing mainly fructose is used for the DHA production by the microalga. The lipid content in biomass, DHA content in total fatty acids, DHA yield, and DHA productivity at 72 h reach 45.26%, 35.59%, 5.64 g L?1 and 1.88 g L?1 d?1, respectively. The results suggest that inulin is an excellent feedstock for Schizochytrium sp. suitable for commercial DHA production. Practical Applications: DHA is an essential nutrient for human health and is widely used in infant formula and functional food. As a reserve carbohydrate, inulin present in plants represents a cheap, abundant, and readily available bioresource. This study describes the suitability of inulin as a promising alternative to glucose for DHA production by Schizochytrium sp. Hence, a practical bioprocess for commercial DHA production from inulin by Schizochytrium sp. could be developed. As far as it is known, this is the first report of inulin as a feedstock for Schizochytrium sp. to produce DHA. |
