薏苡仁多糖的提取及分离纯化

在单因素试验基础上，选取料液比、提取温度和提取时间3 项为考察因素，通过正交试验L9(33)优化薏苡仁多糖提取的最佳工艺条件；对薏苡仁多糖进行分离纯化，考察薏苡仁多糖的SephadexG-75 凝胶柱层析特性以及纯度鉴定。结果表明：料液比1:15(g/mL)、提取温度100℃、提取时间150min 为最佳提取条件，在最佳条件下薏苡仁多糖得率为5.53%；紫外扫描结果显示薏苡仁多糖中不含核酸和蛋白质。

Isolation and Purification of Polysaccharides from Coix lachryma-jobi L. var. ma-yuen Stapf

In order to maximize the extraction yield of polysaccharides from the seeds of adlay (Coix lachryma-jobi L. var. mayuen Stapf), an orthogonal array design L9(33) was applied to optimize the extraction conditions including raw material/ water as well as extraction temperature and duration, of which the effects on polysaccharide extraction yield were initially dealt with by single factor method. Finally, the extracted polysaccharides were purified on Sephadex G-75 gel column, and the chromatographic characteristics were investigated and purity identification was carried out. Material/water ratio of 1:15 (g/mL), extraction temperature of 100 ℃ and extraction time of 150 min were found optimal, and the extraction yield of polysaccharides was 5.53% under these optimal extraction conditions. The purified polysaccharides were analyzed by UV spectroscopy to contain neither protein nor nucleic acids.