小麦胚活性肽对D-半乳糖衰老模型小鼠抗氧化作用研究

目的：研究不同剂量小麦胚活性肽对D- 半乳糖衰老模型小鼠抗氧化作用。方法：将50 只昆明小鼠随机分为5 组(正常对照组，模型对照组，低、中、高剂量多肽组)，除正常对照组外，其余4 组腹腔注射600mg/(kgbw·d) D- 半乳糖建立衰老模型，多肽组灌胃低、中、高剂量(200、800、1000mg/(kg bw·d)小麦胚活性肽， 实验周期45d，眼球取血处死小鼠，测定血清、脑、肝脏和心脏中超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、总抗氧化能力(T-AOC)和丙二醛(MDA)含量。结果：小麦胚活性肽能显著提高血清中T-AOC、GSH-Px、SOD 活力(P ＜ 0.01)以及心脏中SOD 活力 和 T-AOC(P ＜ 0.01)；也能显著提高脑和肝脏中GSH-Px的活力(P ＜ 0.05)和脑中T-AOC(P ＜ 0.05)；同时使血清和各组织中的MDA 含量显著降低(P ＜ 0.05)。结论：说明小麦胚活性肽有较强的体内抗氧化活性。

Antioxidant Effect of Wheat Germ Bioactive Peptides in D-galactose-induced Aging Mice

Objective: To explore the antioxidant effect of wheat germ bioactive peptides (WGBP) in aging mice induced by D-galactose. Methods: Fifty male Kunming mice were randomly divided into five groups, control group, model group, and WGBP low-, medium- and high-dose groups. Except the control group, all the other groups were subjected to subcutaneous injection of D-galactose solution at a dose of 600 mg/(kg bw·d). Mice were treated with WGBP at doses of 200, 800 mg/(kg bw·d) and 1000 mg/(kg bw·d) once a day for the creation of the WGBP low-, medium- and highdose groups. The activities of SOD and GSH-Px, T-AOC and the content of MDA in serum, brain, liver and heart were determined after a trial period of 45 days. Results: The activities of GSH-Px, SOD and T-AOC exhibited a significant increase (P ＜ 0.01) in serum, and the activities of SOD and T-AOC also exhibited a significant increase in heart (P ＜ 0.01) due to WGBP treatment. The activity of GSH-Px in brain and liver exhibited an obvious increase (P ＜ 0.05); meanwhile, an obvious increase of SOD activity in brain was also observed (P ＜ 0.05). Furthermore, the content of MDA in serum and the above organs revealed a reducing trend (P ＜ 0.05). Conclusion: WGBP has good antioxidant effect in vivo as expected.