| 无花果蛋白酶在双水相体系中分配行为 |
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| 引用本文： | 冯自立,马 娜.无花果蛋白酶在双水相体系中分配行为[J].食品科学,2010,31(19):67-70. |
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| 作者姓名： | 冯自立 马 娜 |
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| 作者单位： | 1.陕西理工学院 陕西省资源生物重点实验室
2.陕西理工学院物理系 |
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| 基金项目： | 陕西省教育厅重点实验室资助项目(08JK245) |
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| 摘 要： | 双水相萃取(ATPS)是近年来发展起来的新型、廉价酶纯化方法。为了扩展该方法应用领域，对PEG(polyethylene glycol)/(NH4)2SO4 双水相体系萃取无花果叶中无花果蛋白酶的实验条件进行研究，考察PEG 相对分子质量、成相物质量分数、pH 值、温度对无花果蛋白酶在两相体系中分配系数(K 值)的影响。结果表明：当两相体系中PEG 相对分子质量为1000、PEG 质量分数为20%、(NH4)2SO4 质量分数为19%、pH 值为6、温度室温(25℃)时蛋白酶在两相体系中分配系数最高可达3.6。
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| 关 键 词： | 无花果蛋白酶 双水相体系 分配系数 |
| 收稿时间： | 2009-10-11 |
Partitional Behavior of Ficin in PEG/(NH4)2SO4 Two-phase Aqueous System |
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| FENG Zi-li,MA Na.Partitional Behavior of Ficin in PEG/(NH4)2SO4 Two-phase Aqueous System[J].Food Science,2010,31(19):67-70. |
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| Authors: | FENG Zi-li MA Na |
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| Affiliation: | 1. Bio-resources Key Laboratory of Shaanxi Province, Shaanxi University of Technology, Hanzhong 723001, China；
2. Department of Physics, Shaanxi University of Technology, Hanzhong 723001, China |
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| Abstract: | Aqueous two-phase system (ATPS) was a new and cheap purification method for ficin. The partition of Ficin in polyethylene glycol and ammonium sulfate aqueous two-phase systems was investigated. The effects of PEG molecular weight, polyethylene glycol concentration and ammonium sulfate concentration, pH and temperature on ficin partition coefficient were also explored. Results exhibited that ficin partition coefficient in two-phase aqueous system at the conditions of 20% PEG1000, 19% (NH4)2SO4, pH 6 and temperature at 25 ℃ was 3.6. |
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| Keywords: | ficin two-phase aqueous system partition coefficient K |
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