屠宰场生鸡肉肠炎沙门氏菌的污染途径

目的：研究屠宰场生鸡肉肠炎沙门氏菌的主要污染途径。方法：以屠宰场生鸡肉样品为研究对象，在利用多聚酶链反应(PCR)检测肠炎沙门氏菌污染的基础上，通过流行病学的方法研究生鸡肉污染与肉鸡感染的关系。结果：屠宰场生鸡肉样品肠炎沙门氏菌污染阳性率为9.03%(26/288)，样品污染与样品来源屠宰场间无统计学联系，但与养殖场的肉鸡感染肠炎沙门氏菌有联系；养殖场肉鸡感染肠炎沙门氏菌与生鸡肉污染有正的统计学联系(相对危险性=18.5)；肉鸡实验性感染肠炎沙门氏菌后的带菌时间可达42d，排菌达56d 以上。结论：屠宰场生鸡肉肠炎沙门氏菌的污染途径主要为内源性污染。

Contamination Pathways of Raw Chicken by Salmonella enteritidis in Slaughterhouses

Objective: To explore main contamination pathways of raw chicken by Salmonella enteritidis in slaughterhouses. Methods: The contamination of Salmonella enteritidis in raw chicken samples from different slaughterhouses was detected by polymerase chain reaction (PCR). The relationship between the contamination of raw chicken and the infection of broilers was investigated. Results: A total of 288 samples from 5 slaughterhouses were detected, and the results showed that 9.03% of them were contaminated by Salmonella enteritidis. No significant difference was observed among raw chicken samples from different slaughterhouses; however, the number of contaminated samples was positively correlated with the infection of Salmonella enteritidis during the nursery of broilers (the relative risk was 18.5). The experimental infected broilers harbored Salmonella enteritidis for 42 days and the length of bacteria excretion was approximately 56 days. Conclusion: Endogenous contamination is the predominant contamination pathway by Salmonella enteritidis in raw chicken from slaughterhouses.