可降解咖啡碱菌株的筛选与鉴定

目的：筛选能够降解咖啡碱的细菌，以期在该毒素的生物脱毒中得到应用。方法：以咖啡碱作为唯一碳源和氮源进行咖啡碱降解菌株的初筛，之后将初筛的10 株菌通过液体培养基咖啡碱降解实验最终筛选出降解能力最强的1 株菌。结果：筛选出的HZ-1 菌株在48h 即可将咖啡碱完全降解，对目标菌株细胞形态、生理生化以及16SrDNA 进行鉴定，确定该菌株为Pseudomonas lutea，属于假单胞菌属。结论：利用咖啡碱作为唯一碳源和氮源从土壤中筛选出降解咖啡碱良好的菌株，为脱除咖啡碱提供了微生物菌种，填补了国内空白。

Screening and Identification of a Pseudomonas lutea Strain Capable of Degrading Caffeine

Objective: The aim of this work was to find strains capable of degrading caffeine so as to develop a biological approach for caffeine detoxicification. Method: The preliminary screening of target strains was performed using caffeine as both carbon and nitrogen sources, and then the ten screened strains were further screened by evaluating their abilities to degrade caffeine in liquid medium after culture. As a result, a strain with the strongest ability to degrade caffeine was obtained and named HZ-1. After 48 h culture of HZ-1, caffeine in liquid medium was completely degraded. Based on the morphological, physiological and biochemical properties and 16 rDNA gene sequence, this strain was identified as Pseudomonas lutea. To the best of our knowledge, this is the first report on the biological degradation of caffeine at home.