酶碱法提取枣渣可溶性膳食纤维的工艺研究

以枣渣为原料，采用酶法水解淀粉，碱法水解蛋白质、脂肪的提取方法提取枣渣可溶性膳食纤维，探讨加酶量、酶解时间、碱解pH值、碱解时间、碱解温度等因素对膳食纤维得率的影响。通过正交试验确定了酶碱法制备枣渣可溶性膳食纤维的最佳工艺条件为：糖化酶加酶量为0.4%，纤维素酶加酶量为0.5%、酶解时间60min、碱解pH值为12、碱解温度70℃、碱解时间90min，在此条件下枣渣可溶性膳食纤维得率达11.32%，持水力和溶胀性分别达到848.68%和9.26ml/g。

Enzymolysis Followed by Alkaline Hydrolysis for Preparing Water Soluble Dietary Fiber from Jujube Fruit Residues

In order to develop an optimal preparation procedure of water soluble dietary fiber from jujube fruit residues by a combined technology of enzymolysis with glucoamylase and cellulase and alkaline hydrolysis, single factor and orthogonal array design experiments were carried out. Results showed that the optimum preparation procedure was based on 60 min enzymolysis with a glucoamylase dose of 0.4% and a cellulase dose of 0.5% and 90 min alkaline hydrolysis at pH 12, 70 ℃; under such conditions, the yield of dietary fiber reached 11.23%, and the water holding capacity and swelling capacity of dietary fiber product were 848.68% and 9.26 ml/g, respectively.