| 一株产抗菌活性物质解淀粉芽孢杆菌的筛选及鉴定 |
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| 引用本文： | 杨胜远,韦 锦,李 云,姚 虹,黄月丹.一株产抗菌活性物质解淀粉芽孢杆菌的筛选及鉴定[J].食品科学,2010,31(21):208-212. |
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| 作者姓名： | 杨胜远 韦 锦 李 云 姚 虹 黄月丹 |
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| 作者单位： | 1.韩山师范学院生物系
2.韩山师范学院图书馆 |
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| 基金项目： | 广东省自然科学基金项目(8452104101001546)；国家级星火计划项目(2008GA780032)；韩山师范学院科研基金项目 |
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| 摘 要： | 从菜园土壤中分离筛选到1 株产广谱抗菌活性物质的菌株K6，其抗菌活性物质对藤黄微球菌、大肠杆菌、铜绿假单孢菌、枯草芽孢杆菌、蜡样芽孢杆菌、巨大芽孢杆菌、苏云金芽孢杆菌、酿酒酵母、匍枝根霉和白色念珠菌均有抑制作用，其中对革兰氏阳性菌的抑菌作用最强，对霉菌的抑制作用相对较弱。通过形态特征、16SrDNA 序列分析和系统发育分析，菌株K6 与B. amyloliquefaciens 位于同一簇群，同源性达99%，将其初步鉴定为解淀粉芽孢杆菌。
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| 关 键 词： | 抗菌活性物质 16S rDNA 系统发育分析 解淀粉芽孢杆菌 |
| 收稿时间： | 2010-02-24 |
Screening and Identification of a Bacillus amyloliquefaciens Strain Producing Antimicrobial Substances |
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| YANG Sheng-yuan,WEI Jin,LI Yun,YAO Hong,HUANG Yue-dan.Screening and Identification of a Bacillus amyloliquefaciens Strain Producing Antimicrobial Substances[J].Food Science,2010,31(21):208-212. |
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| Authors: | YANG Sheng-yuan WEI Jin LI Yun YAO Hong HUANG Yue-dan |
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| Affiliation: | 1. Department of Biology, Hanshan Normal University, Chaozhou 521041, China ；
2. Library of Hanshan Normal University, Chaozhou 521041, China |
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| Abstract: | A strain producing broad-spectrum antimicrobial substances, named K6, was isolated and screened from the soil of a local vegetable garden in Xiangqiao district, Chaozhou city, which presented an inhibition effect on the growth of Mirococcus luteus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus thuringiensis, Saccharomyces cerevisiae, Rhizopus stolonifer and Candida albicans. The strongest inhibition effect on gram-positive bacteria was observed. Base on the morphological, 16S rDNA sequence and phylogenic analysis, the strain K6 was classified as Bacillus amyloliquefaciens. |
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| Keywords: | antimicrobial substance 16S rDNA phylogenic analysis Bacillus amyloliquefaciens |
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