沙苑子提取物中多酚含量的测定

研究Folin-Ciocalteu 比色法测定沙苑子中多酚含量的优化条件，并以优化的条件测定沙苑子不同溶剂提取物中多酚的含量。结果表明：Folin-Ciocalteu 显色剂(经稀释10 倍)用量1.00mL、7.5g/100mL Na2CO3 用量3.00mL、反应温度25℃、反应时间120min、测定波长647nm 为沙苑子中多酚含量测定的最佳条件，所用没食子酸标准品在10～100μg/mL 范围内与吸光度呈良好的线性关系(y=0.0081x － 0.0249，R2=0.9996)；沙苑子甲醇、乙醇和丙酮溶液提取物的质量浓度为1g/100mL 左右时较适合测定其多酚的含量。

Determination of Polyphenols in Different Organic Solvent Extracts of Semen Astragali complanati

In order to provide an optimal Folin-Ciocalteu assay for determining polyphenols in different organic solvent (ethanol aqueous solution, acetone aqueous solution and methanol aqueous solution all at 60% cocentration) extracts of Semen Astragali complanati, some Folin-Ciocalteu assay conditions were optimized. The results indicated that the optimal conditions for the determination of polyphenols by Folin-Ciocalteu were as follows: 10-fold diluted Folin-Ciocalteu reagent amount 1.00 mL, 7.5 g/100 mL Na2CO3 amount 3 mL, reaction temperature 25 ℃, reaction time 120 min, and determination wavelength 647 nm. A good linear relationship was observed between absorbance and gallic acid concentration within the range of 10－100 μg/mL (y = 0.0081x －0.0249, R2 = 0.9996). In addition, accurate contents of polyphenols in methanol extract, ethanol extract and acetone extract of Semen Astragali complanati diluted to a concentration of 1 g/100 mL was determined by this method.