超声和Alcalase 酶复合处理水解大豆分离蛋白工艺研究

以大豆分离蛋白为底物，通过单因素试验和正交试验，确定超声和Alcalase 酶复合处理对大豆分离蛋白水解的最佳条件。结果表明，最佳水解条件为大豆分离蛋白质量分数5.0%、超声处理时间30min、加酶量5.0%、酶解pH8.0、酶解温度55℃、酶解时间4.0h，在此条件下，大豆分离蛋白水解度为12.21%。

Ultrasonic-assisted Alcalase Hydrolysis of Soybean Protein Isolate

In this study, soybean protein isolate (SPI) was pretreated by ultrasonic prior to alcalase hydrolysis. Single factor and orthogonal array design methods were employed to deal with the effects of length of ultrasonic treatment and some hydrolysis conditions (substrate concentration, enzyme/substrate ratio, hydrolysis temperature, initial pH and hydrolysis duration) on the degree of hydrolysis (DH) of SPI. The results showed that a maximum DH of 12.21% was obtained when 5.0% SPI was first subjected to ultrasonic pretreatment for 30 min, followed by hydrolysis at pH 8.0 and 55 ℃ for 4.0 h with alcalase added at 5.0% (on the basis of substrate amount).