方波脉冲电穿孔法提高酵母菌细胞通透性的条件优化

为了采用电穿孔法提高酵母菌细胞的通透性，将通过离心得到的酵母菌制成1 × 108CFU/mL 的菌悬液，采用不同的方波电脉冲条件对其进行处理。结果表明：在0.6mol/L 的蔗糖等渗电脉冲介质中，当酵母菌细胞的处理条件为电场强度7kV/cm，脉冲宽度50μs，脉冲数14，间隔时间2s 时，细胞的存活率平均为81.16%，电穿孔率平均为65.14%。这表明电穿孔法可以有效增强酵母菌细胞的通透性。

Optimization of Square-wave Electroporation for Increasing the Permeability of Yeast Cells

Yeast (Saccharomyces cerevisiae CICC 1012) cells were statically cultured in YEPD medium at 30 ℃ for 24 h and then separated by centrifugation at 3000 r/min after the end of culture and suspended in an electric pulse medium (containing sucrose, Tris-HCl and MgCl2, at pH 7.5) at a cell concentration of 1×108 CFU/mL before square-wave electric pulse treatment under different operating conditions. Electric field strength of 7 kV/cm, pulse duration of 50μs, pulse number of 14, time interval of 2 s, and sucrose concentration of 0.6 mol/L were found optimum. The percentage survival of yeast cells was 81.16% and the percentage of electroporated yeast cells was 65.14% under these optimum conditions. This study suggests that the permeability of yeast cells can be increased by square-wave electroporation.