| 超声波辅助反胶束提取菜籽蛋白后萃研究 |
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| 引用本文： | 王立峰,朱均旺,鞠兴荣,袁 建,何 荣.超声波辅助反胶束提取菜籽蛋白后萃研究[J].食品科学,2009,30(22):206-208. |
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| 作者姓名： | 王立峰 朱均旺 鞠兴荣 袁 建 何 荣 |
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| 作者单位： | 1.南京财经大学食品科学与工程学院，江苏省粮油品质控制及深加工技术重点实验室 2.江南大学食品学院 |
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| 基金项目： | 国家“863”计划项目(2007AA10Z331)；江苏省教育厅自然科学研究项目(08KJB55004)；
南京财经大学重点科研项目 |
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| 摘 要： | 探索一种超声波辅助反胶束法提取菜籽蛋白的后萃方法，即先回收异辛烷，然后用少量KCl 缓冲溶液溶解剩余固形物，再添加适量乙醇，最后利用菜籽蛋白进入水相而分离得到。运用Uniform Design(均匀设计)对各因素的最佳水平范围进行研究，通过SPSS 软件对回归方程的分析得最佳后萃率时各因素的最优组合为酒精2ml，钾离子浓度1.2mol/L，缓冲液25ml、pH9.5，在此工艺条件下，菜籽蛋白的后萃率为59.56%。
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| 关 键 词： | 菜籽蛋白 后萃 均匀设计 |
| 收稿时间： | 2009-06-18 |
Backward Extraction of Rapeseed Protein through Reverse Micelle System Assisted by Sonication |
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| WANG Li-feng,ZHU Jun-wang,JU Xing-rong,YUAN Jian,HE Rong.Backward Extraction of Rapeseed Protein through Reverse Micelle System Assisted by Sonication[J].Food Science,2009,30(22):206-208. |
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| Authors: | WANG Li-feng ZHU Jun-wang JU Xing-rong YUAN Jian HE Rong |
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| Affiliation: | 1. Key Laboratory of Grain and Oils Quality Control and Deep-Utilizing Technology of Jiangsu Province, College of Food Science and
Engineering, Nanjing University of Finance and Economics, Nanjing 210003, China；2. School of Food Science and Technology,
Jiangnan University, Wuxi 214122, China |
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| Abstract: | In order to increase the extraction rate of rapeseed protein, a backward extraction was developed based on reverse micelle system associated with sonication. The backward extraction procedure for rapeseed protein was isooctane recycling, residues dissolving with small amount of KCl buffer solution, appropriate amount of alcohol adding, and then rapeseed protein precipitating in lower aqueous phase. Uniform design was applied to optimize crucial factors for extraction. Optimal condition for backward extraction was achieved through SPSS statistical analysis, which was 2 ml, 1.2 mol/L of K+, 25 ml of KCl buffer solution, and pH 9.5. Extraction rate of rapeseed protein was 59.56% under this optimal backward extraction condition. |
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| Keywords: | rapeseed protein backward extraction uniform design |
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