一种苦荞抗真菌肽的纯化及抑菌活性分析

以苦荞种子为材料，经提取、热处理，Resource S 阳离子交换层析及Superdex Peptide 分子筛层析，纯化具有抗真菌活性的多肽。Tricine-SDS-PAGE 分析表明，该多肽的表观分子质量约为8.0kD。表面增强激光解吸电离飞行时间质谱(SELDI-TOF)显示其实际分子质量为3.909kD。该抗菌肽对白腐菌(Panus conchatus)、绿色木霉(Trichoderma reesei)和链格孢霉(Alternaria alternata)均表现出显著的生长抑制活性。绿色木霉的形态学分析显示：受到苦荞抗真菌肽影响的菌丝生长停滞，分支加剧，基内菌丝端部膨大，原生质凝缩。该抗真菌肽的制备工艺简单，产品热稳定性好，抑菌作用明显。

Purification and Activity of an Antifungal Peptide from the Seeds of Tartary Buckwheat (Fagopyrum tataricum)

An antifungal peptide (AFP) having the ability to protect organisms against fungal invasion was isolated from tartary buckwheat (Fagopyrum tataricum) seeds. The purification procedure involved extraction with 20 mmol/L Tris-HCl buffer, heating treatment, Resource S cation exchange column chromatography, and size exclusion chromatography (SEC) on Superdex peptide column. The apparent molecular weight of the purified peptide was approximately 8.0 kD evaluated by Tricine-SDS-PAGE, and its actual molecular weight was 3.909 kD determined by surface-enhanced laser desorption ionization- time of flight (SELDI-TOF). This peptide showed strong antifungal activity against Panus conchatus, Trichoderma reesei and Alternaria alternate and could cause swelling hyphal tips, hyphal distortion and condensed protoplasm in Trichoderma reesei. The easy preparation, high thermal stability and potent antifungal activity of this antifungal peptide demonstrate its great potential in food industry.