| 一株植物乳杆菌的分离鉴定及其隐蔽质粒的序列分析 |
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| 引用本文： | 都立辉,霍贵成,鞠兴荣,刘 芳.一株植物乳杆菌的分离鉴定及其隐蔽质粒的序列分析[J].食品科学,2010,31(17):236-239. |
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| 作者姓名： | 都立辉 霍贵成 鞠兴荣 刘 芳 |
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| 作者单位： | 1.南京财经大学食品科学与工程学院
2.东北农业大学 乳品科学教育部重点实验室
3.江苏省农业科学院农产品加工研究所 |
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| 基金项目： | 国家“863”计划项目(2006AA10Z344)；南京财经大学科研基金项目(C0837) |
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| 摘 要： | 从传统发酵乳制品中分离到1 株乳酸杆菌，经API 50 CH 细菌鉴定系统和16S rRNA 基因序列分析后鉴定为植物乳杆菌。分析该菌的质粒图谱后发现该菌携带多个质粒，将其中的小质粒pLD1 测序，结果显示该质粒的大小为2112bp，碱基G+C 含量为37.8%，只编码1 个复制蛋白，其中有1 个17bp 的序列重复了13 次。BLAST 发现该质粒同pLP2000 等的同源性在95% 以上，其中复制蛋白同其他质粒存在一些氨基酸差异。pLD1 质粒序列的GenBank 登陆号为NC_012220，并且它可用于构建良好的乳杆菌基因工程载体。
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| 关 键 词： | 植物乳杆菌 隐蔽质粒 序列分析 复制蛋白 |
| 收稿时间： | 2010-01-05 |
Isolation and Identification of a Lactobacillus plantarum Strain and Sequence Analysis of Its Cryptic Plasmid |
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| DU Li-hui,HUO Gui-cheng,JU Xing-rong,LIU Fang.Isolation and Identification of a Lactobacillus plantarum Strain and Sequence Analysis of Its Cryptic Plasmid[J].Food Science,2010,31(17):236-239. |
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| Authors: | DU Li-hui HUO Gui-cheng JU Xing-rong LIU Fang |
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| Affiliation: | 1. College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210003, China；2. Key
Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China ；3. Institute of
Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | A strain isolated from traditionally fermented dairy product was identified as Lactobacillus plantarum based on API 50 CH bacteria identification system and 16S rRNA sequence analysis. Plasmid profile indicated that this strain harbored several plasmids. A small plasmid designated as pLD1 was subjected to sequence analysis. This small plasmid had 2112 bp and G+C content of 37.8%. Meanwhile, a sequence with 17 bp was repeated 13 times in this small plasmid. BLAST results showed that pLD1 plasmid had more than 95% similarity with pLP2000 and that there was a difference in replication protein among this plasmid and others. The accession number of this small plasmid is NC_012220 and it is useful for the construction of genetically engineered vectors. |
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| Keywords: | Lactobacillus plantarum cryptic plasmid sequence analysis replication protein |
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