| 响应曲面法优化琼胶水解条件 |
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| 引用本文： | 杨贤庆,刘 刚,戚 勃,李来好,郝淑贤,刁石强. 响应曲面法优化琼胶水解条件[J]. 食品科学, 2010, 31(20): 173-177. DOI: 10.7506/spkx1002-6630-201020035 |
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| 作者姓名： | 杨贤庆 刘 刚 戚 勃 李来好 郝淑贤 刁石强 |
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| 作者单位： | 1.中国水产科学研究院南海水产研究所 2.上海海洋大学食品学院 |
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| 基金项目： | 国家“863”计划项目(2007AA10Z345)；农业部公益性行业(农业)科研专项(200903030-E)；广东省海洋渔业科技推广专项(A200899E01；A200901E02)；国家海洋局海洋公益性行业科研专项(201005020) |
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| 摘 要： | 采用酸水解法对琼胶进行降解并结合苯酚- 硫酸法测定琼胶低聚糖的含量。在单因素试验的基础上，利用响应曲面法优化水解工艺，建立盐酸浓度(X1)、水解时间(X2 ) 和固液比(X3 ) 与水解率( Y ) 之间的数学模型：Y =81.5933＋0.7175X1＋1.8938X2＋2.7313X3－3.1567X12－3.3042X22－3.3092X32－0.6625X1X2＋0.0475X1X3－0.8000X2X3，确定琼胶水解的最佳工艺条件，即盐酸浓度0.105mol/L、水解时间96min、固液比4.5:100(g/mL)，在此最佳工艺条件下进行水解，琼胶水解率为81.43%。
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| 关 键 词： | 琼胶 酸水解 响应曲面法 |
| 收稿时间： | 2010-04-30 |
Optimization of Acid Hydrolysis of Agar by Response Surface Methodology |
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| YANG Xian-qing,LIU Gang,QI Bo,LI Lai-hao,HAO Shu-xian,DIAO Shi-qiang. Optimization of Acid Hydrolysis of Agar by Response Surface Methodology[J]. Food Science, 2010, 31(20): 173-177. DOI: 10.7506/spkx1002-6630-201020035 |
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| Authors: | YANG Xian-qing LIU Gang QI Bo LI Lai-hao HAO Shu-xian DIAO Shi-qiang |
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| Affiliation: | 1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China；2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | Agar was subjected to acid hydrolysis and the content of agar oligosaccharides was determined by hydrochloric acid and phenol-sulfuric acid method. On the basis of single factor experiments, the hydrolysis procedure was optimized using response surface methodology, and a mathematical regression model (Y= 81.5933＋0.7175X1＋1.8938X2＋2.7313X3－3.1567X12－3.3042X22－3.3092X32－0.6625X1X2＋0.0475X1X3－0.8000X2X3) was established for predicting the yield of agar oligosaccharides (Y) at different levels of hydrochloric acid concentration (X1), hydrolysis time (X2), and solid/liquid ratio (X3). The optimal hydrolysis conditions were determined to be hydrochloric acid concentration of 0.105 mol/L, solid/liquid ratio of 4.5:100 (g/mL) and hydrolysis time of 96 min. The yield of agar oligosaccharides was up to 81.43% under the optimal hydrolysis conditions, which was in good agreement with the predicted value. |
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| Keywords: | agar acid hydrolysis response surface methodology |
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