口服酪蛋白复合肽对细胞通讯网络的作用

为了研究酪蛋白复合肽对人体细胞通讯网络的影响，通过青年志愿者服用酪蛋白复合多肽，使用MilliporeHuman Kit 和液体芯片扫描仪检测服用前后血清中32 种细胞因子浓度的变化，结果表明：3 个剂量组共有EGF、GRO、IL-1ra、IL-5、IL-7、IL-17、sCD40L、MIP-1β、TNF-α共9 种细胞因子浓度显著降低。根据9 种显著变化的细胞因子进行信息检索，按照其合成与分泌细胞及其作用的靶细胞数据，构建口服不同剂量酪蛋白复合肽之后机体细胞通讯网络的变化。通过该细胞通讯网络的变化，可以直观地分析服用酪蛋白复合肽前后细胞之间的通讯规律；从细胞通讯网络可以看出酪蛋白复合肽具有下调免疫的作用；其作用的信号途径涉及NF- κB、JNK、JAK/STAT、P38/MAPK、PI-3K/AKT；不同剂量组显著变化的细胞因子不同，细胞通讯规律也不同，说明酪蛋白酶解肽对免疫系统的调节作用具有强剂量相关性。

Effect of Oral Administration of Casein Co-peptide on Human Intracellular Communication Networks

To explore the effect of casein co-peptide on the communication networks among human cytokines, young volunteers recruited orally took three different doses of casein co-peptide after breakfast and lunch each once, and blood was harvested at 3 h after the last administration and changes in the levels of 32 serum cytokines were assayed with Millipore Human Kit or liquid chip scanner. There were 9 serum cytokines exhibiting a significant decrease due to casein co-peptide administration at different doses. Information retrieval was carried out for these cytokines, and based on the data from cells synthesize and secrete these cytokines and their target cells, human intracellular communication networks responding to different doses of casein co-peptide were constructed and compared. The comparative results reveal that casein co-peptide has immune down-regulatory effect. Meanwhile, the signaling pathways of casein co-peptide might involve NF-κB, JNK, JAK/STAT, P38/MAPK and PI-3K/AKT. Different cytokines were changed obviously due to different doses of casein co-peptide, and different intracellular communication patterns were also observed, indicating that the immune regulatory effect of casein co-peptide is in an obvious dose-dependant fashion.