侧柏籽油的超声辅助提取及其脂肪酸组成分析

以环己烷为提取溶剂，采用响应面法(RSM)优化超声波辅助提取侧柏籽油的工艺条件，在单因素试验基础上，选取液料比、提取时间、提取温度、超声波功率为影响因素，以侧柏籽油提取率为响应值，应用中心组合试验设计(central composite design，CCD)建立数学模型，进行响应面分析，并采用GC-MS 测定侧柏籽油的脂肪酸组成。结果表明，提取侧柏籽油的优化工艺条件为：液料比7:1(mL/g)、提取时间38min、提取温度55℃、超声波功率270W，在此工艺条件下，侧柏籽油提取率为93.47%；GC-MS 测定结果表明侧柏籽油中富含不饱和脂肪酸，总含量达到84.37%，其中油酸、亚油酸和亚麻酸的含量分别为28.41%、11.40% 44.56%。

Ultrasound-assisted Extraction and Fatty Acid Composition of Biota Orientalis Seed Oil

Oil in Biota orientalis seed was extracted using n-hexane with the assistance of ultrasound. Effects of liquid-to-material ratio, extraction time, extraction temperature and ultrasound power on the yield of oil were investigated by conducting single factor tests. Subsequently, a central composite design was employed and the results were analyzed by using response surface methodology. The optimal extraction conditions were as follows: liquid-to-material ratio 7:1 , extraction time 38 min, extraction temperature 55 ℃,ultrasound power 270 W. Under such conditions, the yield of the oil was 93.47%. Gas chromatography-mass spectrometry analysis indicated that the oil was abundant in unsaturated fatty acids, accounting for 84.37% of the total fatty acids and consisting of 28.4.1% of oleic acid , 11.40% of linoleic acid and 44.56% of linolenic acid.