高效液相色谱法测定天山花楸中金丝桃苷的含量

目的：建立天山花楸枝叶中金丝桃苷的含量测定方法。方法：采用HPLC Shim-pack VP-ODS 柱(150mm× 4.6mm，5μm)，流动相为乙腈- 水(25:75，V/V)，流速为1.0ml/min，柱温为25℃，检测波长为360nm。结果：金丝桃苷在0.04～0.32mg/ml 之间线性关系良好，r ＝ 0.9992，平均回收率99.7%，RSD 为2.6%；结论：本法快速、灵敏、准确、可靠、重复性好，可用于天山花楸药材的质量控制。

HPLC Determination of Hyperoside in Stems and Leaves of Sorbus tianschanica Rupr

Hyperoside was extracted from stems and leaves of Sorbus tianschanica Rupr with methanol and then subjected to HPLC analysis. The determination (at 360 nm) was performed on a Shim-pack VP-ODS (150 mm × 4.6 mm, 5 μm) column (25 ℃) using aceyonitrile - water (25:75, V/V) as a mobile phase at the flow rate of 1.0 ml/min. A good linearity was obtained within the range of 0.04 － 0.32 mg and the correlation coefficient was 0.9992. The average recovery for hyperoside was 99.7% and the RSD was 2.6%. The method is simple, rapid, sensitive, precise, reliable and repeatable and can be applied to the quality control of stems and leaves of Sorbus tianschanica Rupr as a kind of Chinese medicinal material.