新疆维药阿里红发酵液多糖提取工艺研究

采用单因素和正交试验，以多糖回收率为指标，对阿里红发酵液菌丝体胞内和发酵液胞外多糖提取工艺进行优化。结果表明，阿里红菌丝体胞内多糖提取的最佳工艺为超声波功率90W、超声时间15min、热水浸提温度90℃、干菌丝体与蒸馏水质量比为1:20、浸提时间3h，每克干菌丝体可提取胞内多糖66.4mg。发酵液胞外多糖的最佳提取工艺为乙醇体积分数60%、沉淀温度4℃、发酵液pH 值中性、沉淀时间24h，最高得率可达92.1%。

Optimizing the Extraction Processes of Polysaccharides from Fermented Mycelia of Fermentation Supernatant of Fomes officinalis Ames as a Traditional Uyghur Medicine

The conditions for extracting intracellular polysaccharides from the fermented mycelia of Fomes officinalis Ames that is a traditional Uyghur medicine in Xinjiang autonomous region and extracting extracellular polysaccharides from the fermentation supernatant of the strain were optimized using single factor and orthogonal array design methods. The optimal conditions for the extraction of intracellular polysaccharides under the assistance of ultrasonic were determined to be: ultrasonic power 90 W; ultrasonic treatment time, 15 min; hot water temperature, 90 ℃; dried mycelia/distilled water ratio, 1:20; and length of extraction time, 3 hours, and an intracellular polysaccharide yield of 66.4 mg/g dried mycelia was obtained under such conditions. The optimal extraction process of extracellular polysaccharides consisted of adjusting the pH of the fermentation supernatant to 7 and adding 60% ethanol for polysaccharide precipitation for 24 hour at 4 ℃, and the resultant polysaccharide yield was 92.1%.