| 耐热蛋白酶对UHT乳蛋白水解作用 |
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| 引用本文： | 王 辉,吕加平,刘 鹭,靳 磊.耐热蛋白酶对UHT乳蛋白水解作用[J].食品科学,2010,31(17):228-231. |
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| 作者姓名： | 王 辉 吕加平 刘 鹭 靳 磊 |
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| 作者单位： | 1.中国农业科学院农产品加工研究所，农业部农产品加工与质量控制重点开放实验室
2.国家知识产权局专利审查协作中心 |
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| 基金项目： | 国家科研院所社会公益项目(2002005DIA4G035-7)；“十一五”国家科技支撑计划项目(2006BAD04A07；2006BAD04A10) |
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| 摘 要： | 采用SDS-PAGE、Urea-PAGE 分析耐热蛋白酶对UHT 乳蛋白的水解作用。结果表明：荧光假单胞菌(PF)蛋白酶和纤溶酶(PL)作用底物不同，产物也不同；前者优先水解κ- 酪蛋白生成副κ- 酪蛋白，而PL 主要水解β- 酪蛋白与α- 酪蛋白生成γ- 酪蛋白及胨、肽等。分别经PL和细菌蛋白酶水解3h的UHT乳水解产物，在pH4.6和12g/100mL三氯乙酸(TCA)沉淀后经反相高效液相色潽仪(RP-HPLC)分析滤液，两者呈现不同的色谱图。
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| 关 键 词： | 耐热蛋白酶 UHT乳 水解 |
| 收稿时间： | 2009-12-18 |
Hydrolysis Effect of Heat-resistant Protease on UHT Milk |
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| WANG Hui,LU Jia-ping,LIU Lu,JIN Lei.Hydrolysis Effect of Heat-resistant Protease on UHT Milk[J].Food Science,2010,31(17):228-231. |
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| Authors: | WANG Hui LU Jia-ping LIU Lu JIN Lei |
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| Affiliation: | 1. Institute of Agro-food Science and Technology, Key Laboratory of Agricultural Product Processing and Quality Control, Ministry of
Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100193, China；2. Patent Examination Cooperation Center of State
Intellectual Property Office, Beijing 100190, China |
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| Abstract: | The effect of heat-resistant protease on protein proteolysis of UHT milk was evaluated by SDS-PAGE and Urea- PAGE. Results indicated that different proteases reacted with different substrates in milk to produce different peptides. Pseudomonas fluorescens protease hydrolyzed κ -casein into para- κ -casein. Plasminogen hydrolyzed α -casein and β -casein into γ-casein, peptone and peptides. In addition, the leftover supernatants after trichloroacetic acid precipitation of the 3 h hydrolysates of UHT milk prepared with plasminogen and bacterial proteases exhibited different RP-HPLC profiles and those of the pH 4.6 soluble peptide in the 3 h hyolroluysates were also different. |
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| Keywords: | heat-resistant protease UHT milk proteolysis |
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