气相色谱- 质谱法测定蜂王浆中的植物甾醇组成

为探明蜂王浆中的植物甾醇组成，蜂王浆样品经冻干、萃取得到王浆脂类物质，再经过皂化、衍生后使用HP-5MS 柱进行分离，采用GC-MS 对蜂王浆中甾醇类物质进行鉴定，并用GC-FID 内标法进行定量分析。结果表明：蜂王浆中除含有少量胆固醇外，还含有25- 羟基-24- 甲基胆甾醇、菜油甾醇、豆甾醇、β - 谷甾醇、Δ5-燕麦甾醇、Δ7- 燕麦甾醇等植物甾醇；蜂王浆中总甾醇含量在51mg/100g 鲜王浆以上。其中，25- 羟基-24- 甲基胆甾醇、菜油甾醇、β- 谷甾醇和Δ5- 燕麦甾醇含量较高，分别占总甾醇含量的36.79%、6.30%、19.75% 和27.99%。

Sterol Composition Analysis of Royal Jelly by Gas Chromatography Coupled with Mass Spectrometry

To figure out the sterol composition of royal jelly, royal jelly samples were lyophilized and extracted and the extracted lipid substances were then saponified, derivatized and separated on a HP-5MS column prior to gas chromatography with flame ionization detection (GC-FID ) coupled with mass spectrometric identification of sterols, and quantitative analysis was finally conducted by internal standard method. A small amount of cholesterols was contained in royal jelly, and the main sterols were 25-hydroxy-24-methylcholesterol, campesterol, stigmasterol, β-sitosterol, Δ5-avenasterol and Δ7-avenasterol. The total content of sterols in 100 g of fresh royal jelly was higher than 51 mg. Among these sterols, the most predominant components were 25-hydroxy-24-methylcholesterol, campesterol, β-sitosterol and Δ5-avenasterol, accounting for 36.79%, 6.30%, 19.75% and 27.99%, respectively.