| 同时检测恩诺沙星和环丙沙星单克隆抗体的制备 |
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| 引用本文： | 郭杰标,许 杨,刘师文. 同时检测恩诺沙星和环丙沙星单克隆抗体的制备[J]. 食品科学, 2010, 31(13): 205-208. DOI: 10.7506/spkx1002-6630-201013047 |
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| 作者姓名： | 郭杰标 许 杨 刘师文 |
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| 作者单位： | 南昌大学 食品科学与技术国家重点实验室，中德联合研究院 |
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| 基金项目： | 江西省社会发展科技攻关计划项目(2007BS12501) |
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| 摘 要： | 为了产生同时检测恩诺沙星和环丙沙星的抗体，本研究采用戊二醛法制备突出恩诺沙星和环丙沙星共同结构的人工抗原。免疫Balb/c 小鼠的结果显示：使用糖基化载体所制备的免疫抗原，能够大幅提高所产生特异性抗体的滴度。通过细胞融合，筛选出一株对恩诺沙星和环丙沙星IC50 值分别为9.6ng/mL 和10.2ng/mL 的单克隆抗体。
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| 关 键 词： | 恩诺沙星 环丙沙星 糖基化载体 兽药残留 人工抗原 免疫效果 |
| 收稿时间： | 2009-12-16 |
Preparation of Monoclonal Antibody for Simultaneous Detection of Enrofloxacin and Ciprofloxacin |
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| GUO Jie-biao,XU Yang,LIU Shi-wen. Preparation of Monoclonal Antibody for Simultaneous Detection of Enrofloxacin and Ciprofloxacin[J]. Food Science, 2010, 31(13): 205-208. DOI: 10.7506/spkx1002-6630-201013047 |
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| Authors: | GUO Jie-biao XU Yang LIU Shi-wen |
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| Affiliation: | State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute,Nanchang University, Nanchang 330047, China |
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| Abstract: | In order to generate a monoclonal antibody with specificity to simultaneously detect enrofloxacin and ciprofloxacin, glutaraldehyde was used to prepare the artificial antigens with common structures of enrofloxacin and ciprofloxacin exposed as the major epitopes. Balb/c mice were immunized with the prepared immunogens. Results indicated that glycosylated BSA used for immunogen synthesis resulted in a higher antiserum titer when compared to unglycosylated BSA. A monoclonal antibody with respective IC50 values of 9.6 ng/mL and 10.2 ng/mL to enrofloxacin and ciprofloxacin was successfully prepared through cell fusion. |
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| Keywords: | enrofloxacin ciprofloxacin glycosylated carrier veterinary drug residues artificial antigen immunological effect |
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